【摘 要】
：
Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through
【机 构】
：
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi,PR Ch
【出 处】
：
2011中国肉牛选育改良与产业发展国际研讨会
论文部分内容阅读
Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS).The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes.We have cloned the cDNA sequence coding UQCC gene in bovine.Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC.Furthermore, Real-Time PCR analysis show that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues.We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs.SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW).For the A2691T SNP marker, there are significant effects on the RL (p =0.0001), CD (p =0.0059) and PBW(p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p =0.0047) and CD (p =0.0454.Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p =0.0215), CD (p =0.0282) and PBW (p =0.0329) in the total population.The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing of an animal model using cattle to study big animal body type.
其他文献
GPR54 has been implicated in the neuroendocrine regulation of GnRH secretion.To determine the polymorphism of GPR54 gene and its association with sexual maturity in cattle,Simmental with early sexual
The AMPD1 gene plays an important role in the purine nucleotide cycle and energy metabolism in skeletal muscle.In the present study, polymorphism of the AMPD1 gene was detected by PCR-SSCP and DNA seq
Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef,with its endogenous synthesis regulated by Stearoyl CoA desaturase (SCD1) and diacylglycerol-acyltransferasel (
To determine the genetic diversity and paternal origin of Chinese cattle, 284 male samples from 16 Chinese native cattle breeds were analyzed using four previously defined Y-SNP markers that distingui
In the present study we have examined whether p38 mitogen activated protein kinase (p38MAPK) signaling pathway interacts with calcium signaling on lipid accumulation in primary preadipocytes of mice.T
OLR1 is involved in fatty acid transport and binds and degrades the oxidized form of low-density lipoprotein.The objective of the study was to identify the association of gene diversity with milk perf
In order to find genetic markers of productive traits in Grassland Red Cattle(GLRC), the relationship between 5 microsatellite loci (DVGA2, DVGA46, DVGA44, BM2113 and TGLA44) and21 performance traits
Introduction Queensland (QLD) is Australias second largest state spreading over 1,730,648 km2, which is about 22.5％ of the Australian continent.Queensland stands as the biggest producer and exporter o
The aim of the present study was to verify associations between bull meat traits and six microsatellite loci.A sample of 66 weaned young bulls from Grassland Red Cattle (GLRC) and its improved hybrids
Leptin gene plays an important role in the regulation of feed intake, fat deposition, energy metabolism, fertility and immune functions.This study was aimed at investigating the effects of leptin gene