Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm

• 作 者: YongHou[1]DiShao[2]HanjieWu[2]XiaofeiYe[2]ChenYe[2]RenhuaWu[2]MinJian[2]YanChen[3]WeiXie[4]RurenZhang[4]LeiChen[5]LutingSong[6]XinLiu[2]XiaotianYao[2]HanchengZheng[2]ChangYu[2]QibinLi[2]ZhuolinGong[2]
• 机 构: BGI-Shenzhen, Shenzhen, 518083, China
• 出 处: 第八届全国难治性白血病学术研讨会、第四届全国难治性淋巴瘤学术研讨会、第四届全国多发性骨髓瘤学术研讨会、第五届钟山国际MD
• 发表日期: 2012年期

　　Tumor heterogeneity presents a challenge for infer ring clonal evolution and driver gene identification.Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level.To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell se quencing method using two lymphoblastoid cell line single cells.We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient.The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution.