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In this study,regenerated silk fibroin (RSF) fibers were obtained by extruding of concentrated silk fibroin aqueous solution into ammonium sulphate coagulation bath.A new custom-made industrial wet-spinning device with continuous mechanical post-drawn and a biomimetic spinning nozzle was used in our trial spinning.The breaking stress and the breaking stress of RSF fibers was respectively 0.23 Gpa and 38.3％.