Vaccination with a co-expression DNA plasmid containing GAD65 fragment gene and IL-10 gene Induces R

• 作 者: Xinyuan Liu Song Zhang Xia Li Peilin Zheng Fang Hu Zhiguang Zhou
• 机 构: InstitutionofMetablismandendocrinology,SecondXiangyaHospital,CentralSouthUniversity,Changsha410011,C
• 出 处: 第十届湘雅国际糖尿病免疫学论坛
• 发表日期: 2015年4期

OBJECTIVE: The non-obese diabetic (NOD) mouse strain spontaneously develops a disease similar to human Type 1 diabetes (T1D).The aims of our study are to explore the potential mechanisms of an optimized co-expression DNA vaccine containing GAD65 fragment gene and IL-10 gene(SGAD65190-315/IL-10) preventive effect in NOD mice.METHODS: Female NOD mice at 3-4 weeks of age were randomly divided into 2 groups and received intra-muscular injection of either blank vector (n=34) or pBudCE4.1 carrying the SGAD65190-315/IL-10 (n=32).The incidence of diabetes was monitored up to 30 weeks of age.The severity of insulitis, β cells apoptosis rate, and relevant mechanisms were examined.