【摘 要】
：
In clinical proteomics study, serum and plasma are frequently used for the detection of early diagnostic biomarkers.Although mass spectrometry (MS) based pr
【机 构】
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Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
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In clinical proteomics study, serum and plasma are frequently used for the detection of early diagnostic biomarkers.Although mass spectrometry (MS) based proteomic research has played an important role in discovering potential candidate biomarkers, now available methods, such as offline 2-DE MS/MS and on-line 2D-LC-ESI-MS/MS, are still of great challenges due to the complexity of proteome.Therefore, powerful protein prefractionation methods, such as high-abundance removal by antibody techniques or multidimensional LC, are often adopted.To achieve MS based protein identification, protein pretreatment including denaturation, reduction, alkylation, desalting and digestion should be performed, which are not only tedious and time-consuming, but also might suffer from sample loss and contamination (e.g., keratins).
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