Quantification of Matrix Metalloprotease-9 in Bronchoalveolar Lavage Fluid by Selected Reaction Moni

• 作 者: LauretteM.Prely[1]KrisztinaPaal[1]JosHermans[1]SiccovanderHeide[2]AntoonJ.M.vanOosterhout[2]RainerBischoff[1]
• 机 构: Analytical Biochemistry, Department of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 97
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　Quantitative protein analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the selected reaction monitoring (SRM) mode is developing into a major alternative to current immunochemical assays.In order to quantify human matrix metalloprotease-9 (MMP-9) in bronchoalveolar lavage fluid (BALF) from patients having undergone lung transplantation, we developed an SRM assay for microfluidics-based nanoLC-MS/MS on a triple quadrupole mass spectrometer based on two signature peptides: AVIDDAFAR and AFALWSAVTPLTFTR.