IDENTIFICATION OF THREE VIBRIO ALGINOLYTICUS STRAINS FROM DIFFERENT SOURCES AND THEIR TOXINS

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  Vibrio alginolyticus can cause acute diarrhea and food poisoning like Vibrio parahemolyticus.In this study,three strains of V.alginolyticus (No.PCG01,02,03) from different sources were first identified with molecular biological method according to hsp60 gene of them.Then the three strains were inspected for their toxins with a series of methods:blood agar plate,rabbits small intestine ligation and suckling mouse model.The result revealed that the strains of PCG 01 and 03 could produce heat-labile enterotoxin (LT),and the strains of PCG01 and 02 have the activity of hemolysin.It was found that the strains of PCG 01 and 03 were positive to produce heat-stable enterotoxin (ST) and the other strain was suspectable to produce ST.Therefore,V.alginolyticus from different sources did have different capability to produce toxins.
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