Liver,contributes to upregulation of haptoglobin in serum from rats with traumatic brain injury

• 作 者: Shu-GuangYANG;Yan-HongMA;YongLIU;Chang-QiangZHU;MengJIANG;Shao-JunLIU;
• 机 构: State Key Laboratory of Proteomics and Department of Neurobiology, Institute of Basic Medical Scienc
• 出 处: 中国神经科学学会第九届全国学术会议暨第五届会员代表大会
• 发表日期: 2011年期

　　Proteomic analysis, including two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry (MS), was used to compare the protein profile of serum samples from rats with traumatic brain injury (TBI) and normal subjects.Haptoglobin (Hp), the acute phase response protein, showed an increased expression in rats with TBI versus normal and its change in serum was confirmed by Western blot.Furthermore, we found that Hp was mainly expressed in liver of rats with TBI and had an elevated mRNA level.To further determine the source of the increased Hp in serum, in vitro studies using HL7702 lines and in vivo studies were performed.The results showed that Hp gene expression was also upregulated in HL7702 lines and liver treated with serum and brain homogenate from rats with TBI.These findings suggest that the increased Hp in serum may result from overexpression of Hp in liver and modulated by a positive feedback loop between brain, blood and liver in response to TBI.