论文部分内容阅读
A method for the simultaneous separation and determination of glyoxalate and oxalate was developed by capillary zone electrophoresis (CZE).Operation parameters including type, concentration and pH of running buffer and applied voltage were investigated.An uncoated fused-silica capillary with a total length of 55cm (effective length 38cm) ×50μm i.d.was used as the separation channel.The best results were obtained by using 20 mmol/L borax-5.5 mmol/L potassium hydrogen phthalate (pH 9.0), applied voltage of 20 kV, and detected wavelength of 212 nm.The optimized method was validated for linearity, precision and accuracy and proved to be robust.The limits of detection of glyoxalate and oxalate were 0.2 and 0.06 mg/mL, respectively.The recoveries ranged from 98.1 to 101.9% with acceptable relative standard deviations (RSDs) less than 0.74%.Its suitable for quantitative and qualitative analysis of glyoxalate and oxalate.