【摘 要】
：
AIM Our present study aimed to test the anti-cancer effect of natural product ZX-1201 on pancreatic cancer cell PANC-1.METHODS After incubation with different concentrations of drug or vehicle, PANC-1
【机 构】
：
State Key Laboratory of Natural & Biomimetic Drugs,Department of Pharmacology,School of Basic Medica
【出 处】
：
第二届世界天然药物和传统药物药理学学术会议
论文部分内容阅读
AIM Our present study aimed to test the anti-cancer effect of natural product ZX-1201 on pancreatic cancer cell PANC-1.METHODS After incubation with different concentrations of drug or vehicle, PANC-1 cell viability was measured by MTS assay, and Scratch assay and Transwell assay were utilized to detect PANC-1 cell migration.Meanwhile, morphological changes were recorded by light microscope.Real-time PCR, immunofluorescence and Western blot were used to examine change of aquaporin-1 (AQP1) and TGF-β1 related EMT pathways.In addition, we performed AQP1 and TGF-β1 RNAi and over-expression experiments to elucidate their role in anti migratiou effect of ZX-1201.RESULTS We found ZX-1201 showed significant inhibitory effect on cell migration in a dose-dependent manner, however, slight cell viability suppression was observed.Next, we demonstrated ZX-1201 could prevent PANC-1 cell from epithelial-mesenehymal transition (EMT), by reducing mesenehymal phenotype, decrease AQP1 and TGF-β1 expression, and dramatically down-regulating EMT markers and signaling pathway.Eventually, we verified that ZX-1201 reversed EMT by targeting AQP1 and TGF-β1, because either AQP1 or TGF-β1 over-expression abolished the inhibitory effect of ZX-1201 on PANC-1.CONCLUSION ZX-1201 significantly inhibits pancreatic cancer cell PANC-1 EMT process through an AQP1 and TGF-β1 targeting manner.
其他文献
AIM We have demonstrated for the first time that nobiletin (NB) from citrus peels improves memory impairment in Alzheimers disease (AD) model animals.METHODS AND RESULTS Amyloid precursor protein (APP
AIM Lycium barbarum L.is a renowned Yin strengthening agent in traditional Chinese medicine.Lycium barbarum L.polysaccharide-protein complex is well-known for its immunoregulatory and antitumor effect
China is one of the oldest and largest countries on the application of natural herbal in the world and have more available medicinal resources.Numerous clinical experiencefrom the combined treatmentof
Artesunate is a semi-synthetic derivative of artemisinin, an active component of the medicinal plant Artemisia annua.Artesunate is a clinically effective anti-malarial drug.Allergy is an acquired hype
AIM Botanical preparations are widely used by patient with cancer in Korea, Japan and China.Rhus verniciflua Stokes (RVS) has traditionally been used as a medicinal ingredient for the therapy of stoma
AIM Kangen-karyu (Guan-Yuan-Ke-Li; KK) is a Chinese prescription consisting of six crude drugs such as Salviae Miltiorrhizae Radix.KK reportedly inhibits platelet aggregation and protects cognitive fu
AIM Up to now, the only currently approved treatment for acute ischemic stroke (AIS) is alteplase, a thrombolytic agent given intravenously within 4.5 h of symptom onset, in an attempt to re-open occl
AIM To further investigate the new binding mode and action of mechanism of a novel IKK-β inhibitor which can specifically interact with new site on IKK-β and then suppress inflammatory conditions in v
Cardiovascular diseases (CVDs) are the leading causes of death and disability in the world.CVDs have emerged as a major health threat in developing countries contrary to the prior belief that they wer
AIM 20 (s)-protopanaxadiol (PPD) is the metabolites of Ginseng saponin, including in both American ginseng (Panax quinquefolius L., Araliaceae) and Asian ginseng (Panax ginseng C.A.Meyer).In our previ