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目的观察天麻素对化疗痛大鼠的治疗作用,并观察大鼠脊髓背角腰段GFAP表达的变化,研究其在脊髓水平的作用机制。方法用长春新碱隔日腹腔注射制作大鼠化疗痛模型,模型成功制备后腹腔注射天麻素治疗,用电子Von Frey测痛仪测定大鼠机械痛阈值,电子热板仪测定大鼠热刺激痛阈值;采用免疫组化方法检测脊髓背角星形胶质细胞特异性活化标志物GFAP的表达。结果实验第10天化疗痛大鼠模型成功建立,用不同剂量天麻素治疗模型大鼠1～2周后,治疗组大鼠的机械和热刺激痛阈值与模型组比较均有不同程度的提高,且呈剂量依赖性,以大剂量天麻素的作用最明显(P<0.01);模型组的脊髓背角灰质中可见GFAP表达明显增强,提示星型胶质细胞明显活化,而治疗组与模型组比较明显减弱。结论天麻素可减轻长春新碱诱导的大鼠化疗痛反应;其作用机制可能与在脊髓水平抑制星型胶质细胞活化有关。
Objective To observe the therapeutic effect of gastrodin on rats with chemical pain and to observe the changes of GFAP expression in the spinal dorsal horn of spinal cord in rats and its mechanism at spinal level. Methods The rat model of chemotherapeutic pain was established by intraperitoneal injection of vincristine on the next day. The model was successfully prepared by intraperitoneal injection of gastrodin. The threshold of mechanical pain was measured by electronic Von Frey apparatus. The thermal stimulus threshold Immunohistochemistry was used to detect the expression of glial cell-specific activation marker GFAP in spinal dorsal horn. Results The rat model of chemotherapy-induced pain was successfully established on the 10th day of experiment. After treated with different doses of gastrodin for 1 to 2 weeks, the pain threshold of mechanical and thermal stimulation in the treatment group increased to some extent compared with the model group, (P <0.01). In the model group, GFAP expression was significantly increased in dorsal horn of spinal cord, suggesting that astrocytes obviously activated, while the treatment group and model group Obviously weakened. Conclusion Gastrodin can attenuate vincristine-induced chemotherapy-induced pain reaction in rats. The mechanism may be related to the inhibition of astrocyte activation at spinal level.