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Flower-like CuO and flower-like CuO/graphene composite were prepared successfully by hydrothermal method.They were characterized by by X-ray diffraction, scanning electron microscopy, nitrogen adsorption, temperature-programmed reduction, and thermogravimetric analysis.It was found that the flower-like CuO microspheres, which are composed of CuO nanosheets, possess an average diameter of 4.2 μm and a Brunauer-Emmett-Teller surface area of 12.6 m2 g-1.Compared to the flower-like CuO, the obtained flower-like CuO/graphene composite shows an enhanced electrochemical performance with a higher capacity of 603 mAh g-1 at 0.1 C rate and 382 mAh g-1 at 1 C rate, and exhibits a better cycle stability with high capacity retention of 95.5 % after 50 cycles even though at 1 C rate.Therefore the flower-like CuO/graphene composite is a potential anode material in Li-ion batteries.