Identification of a novel class of seed development-related proline-rich protein genes in Wheat

• 作 者: HaiyanJia;ShirongLiu;ShengweiMa;LiMa;ZhengqiangMa;
• 机 构: The Applied Plant Genomics Lab,Crop Genomics and Bioinformatics Center,Nanjing Agricultural Universi
• 出 处: 第六届全国小麦基因组学及分子育种大会
• 发表日期: 2015年期

　　Yield formation of plants are governed by a large network of genes under complicated regulation,for which our understanding is still at the infant stage.In this study,a novel class of seed development-related proline-rich protein(PRP)genes,consisting of at least four homologs,was identified in wheat.Structurally,they are distinct from the previously described five classes of plant PRPs.Semi-quantitative RT-PCR showed that these wheat PRPs were mainly expressed in reproductive organs,and their expression levels were reduced gradually as the seeds grew.One of these genes,TaPRP.1,were fused to a Actinl promoter and transformed into a wheat cultivar through particle bombardment.The transgenic lines had significantly higher unit grain weight than the non-transgenic control in three different trials and showed a higher level of TaPRP.1 expression.