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Mass spectrometric imaging(MSI)provides an attractive opportunity to detect and probe the molecular content of tissues in an anatomical context.This technique creates distribution maps of select compounds without the need for priori knowledge of target analytes.In this presentation,I will describe our efforts and recent progress in mapping and imaging of a wide variety of signaling molecules in several biological systems,highlighting the unique challenges and important roles of MSI in the areas of proteomics,peptidomics and metabolomics.