Anti-liver-cancer effect of Scutellaria baicalensis Georgi

• 作 者: Tserentsoo B Tserendagva D Zorig T Oyunsuren Ts Boldbaatar j Dagvatseren B Oldokh S
• 机 构: School of Traditional Medicine,Health Sciences University of Mongolia
• 出 处: 22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
• 发表日期: 2013年7期

Objective: To study effectiveness of Scutellaria baicalensis Georgi.Extract on the liver cancer cells via in vitro.Methods: Ethanol extracts of Scutellaria baicalensis (Georgi)were prepared from its rhizomes and herbs.Primary cancer cells were grown RPMI-1640 containing 10％ fetel bovine serum,MDCK cells in DMEM.Cells were incubated at 37℃ with 95％air and 5％ CO2.DNA ladder assay: We used 6-hole cell culture panel and 3 of them have been cultured with same numberof cells.By using I-genomic DNA extraction kit, we extracted genomic DNA from experimental and control cells, and we analyzed them by 0.8％ agarose gel electrophoresis method and took photos.Bcl-2, ZFYVE1, GAPDH genes are involved in cancer cell division and apoptosis and their gene expression carried out by using RT-PCR in RNA isolated from the experimental and control cells with extract and without extract.RT-PCR products tested with agarose gel and cell genomic products with extract compared with control cells and they were calculated by ImageJ program.