Identification of differentially expressed proteins in the brown alga Hizikia fusiformis under coppe

• 作 者: HuixiZou;QiuyingPang;XiufengYan;
• 机 构: College of Life and Environmental Science,Wenzhou University,Wenzhou 325035
• 出 处: 中国生态学学会海洋生态专业委员会2012年学术年会
• 发表日期: 2012年期

　　Hizikia fusiformis is one of the important commercially cultivated seaweeds in China.The aim of this work was to identify several cellular processes potentially involved in copper stress responses and tolerance in H.fusiformis using a proteomic approach.Algal material was cultivated in seawater without copper (control) or exposed to high copper concentrations for short periods of time,lower sub-lethal copper concentrations for longer periods of time,and the toxicity of copper was assessed by epifluorescence microscopy observations.A significant drop to 30% of chlorophyll autofluorescence was observed under 8h of incubation with 5 mg/L Cu and under 10 days of incubation with 0.5 mg/L Cu,indicating the occurrence of cell death.Therefore,we select this time point to collect samples for proteomic analysis.