【摘 要】
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Ensifer alkalisoli YIC4027T is an α-proteobacterial nitrogen-fixing symbiont of Sesbania cannabina.S.cannabina has been widely used as a green manure for the cultivation and soil remediation,however,i
【机 构】
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Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Rese
【出 处】
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第二十次全国环境微生物学学术研讨会
论文部分内容阅读
Ensifer alkalisoli YIC4027T is an α-proteobacterial nitrogen-fixing symbiont of Sesbania cannabina.S.cannabina has been widely used as a green manure for the cultivation and soil remediation,however,its beneficial symbionts genomic features are pooly understand.In this study,we reported the sequencing and annotation of the E.alkalisoli YIC4027 genome and compared with the other species of Ensifer.Results demonstrated that the 6.1 Mbp genome consists of three circular replicons: one chromosome of 3.7 Mbp and two plasmids,of 1.9 Mbp and 0.46 Mbp,respectively.Phylogenetic analysis based on genomic sequences showed that E.alkalisoli and E.fredii are closely related.Futher synteny analysis of the two species revealed that the much larger chromosome is colinear.An region about 2.3 Mbp ofE.fredii USDA257 chromosome displayed similarity to the megaplasmid of E.alkalisoli YIC4027.Comparative analysis at the genomic level suggested that genes involved in nod factor and protein secretion systems may be responsible for the remarkable difference in host plant range between the two species.Finally,the presented genomic data provides useful information to better understanding the rhizobia-legume symbiosis and the ability that adapt to a saline-alkaline environment.
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