【摘 要】
：
Platinum-based chemotherapy,such as cisplatin,is the primary treatment for ovarian cancer.However,drug resistance has become a major impediment to the successful treatment of ovarian cancer.To date,th
【机 构】
：
VP of R&D, Monarch LifeSciences Proteomics Indiana University USA
【出 处】
：
2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
Platinum-based chemotherapy,such as cisplatin,is the primary treatment for ovarian cancer.However,drug resistance has become a major impediment to the successful treatment of ovarian cancer.To date,the molecular mechanisms of resistance to platinum-based chemotherapy remain unclear.In this study,we applied a LC/MS-based,label-free,protein quantification method to examine the global protein expression profiles of two pairs of ovarian cancer cell lines,A2780/A2780-CP (cisplatin-sensitive / cisplatin-resistant) and 2008/2008-C 13 * 5.25 (cisplatin-sensitive/cisplatin-resistant).We identified and quantified over 2000 proteins from these cell lines and 95 proteins showed significant expression changes between sensitive and resistant groups with a false-discovery-rate (FDR) of less than 5%.Bioinformatics analysis suggested several potential pathways that may be involved in cisplatin resistance.Among these potential pathways,a redox regulated pathway involving superoxide dismutase 1 (SOD1) was targeted in order to further explore its involvement in drug resistance.SOD 1 represents a potential biomarker for early evaluation of drug resistance.Inhibition of SOD 1 activity enabled re-sensitization of the cisplatin resistant ovarian cancer cells.This study provides not only a new proteomic platform for large-scale quantitative protein analysis,but also important information for potential biomarkers of cisplatin resistance in ovarian cancer.Furthermore,these results may be clinically relevant for diagnostics,prognostics,and therapeutic improvement for ovarian cancer treatment.
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