Syntheis of hapten and development of immunoassay based on monoclonal antibody for the detection of

• 作 者: Ping Lu Deyu Hu Song Yang Xiaoyan Ding Mengmeng Shi
• 出 处: 第九届全国磷化学化工学术研讨会
• 发表日期: 2013年11期
• 关键词: Dufulin ELISA immunoassay hapten synthesis

　　An indirect competitive enzyme-linked immunosorbence assay (ic-ELISA) was developed for the quantitative detection of the anti-TMV agent.Hapten 6-[2-((methyllbenzothiazol-2-yl)-1-(2-ethoxy)-O,O-diethyl-α-aminopho-sphonate)acetamido)] hexanoic acid (DHS) was prepared from commercial chemicals and incorporated into the spacer arm through a carbon-carbon single bond.