lsolation of the β- carotene ketolase gene promoter from Haematococcus p luvialis and its functional

• 作 者: 王潮岗 胡章立 赵昌南 毛小茉
• 机 构: 深圳市海洋生物资源与生态环境重点实验室,深圳大学生命科学学院,深圳,518060
• 出 处: 中国藻类学会第八次会员大会暨第十六次学术讨论会
• 发表日期: 2011年11期

@@ Under stress conditions such as high light, heat, salt stresses, and nitrogen-deficiency, the unicellular green alga Haematococcus pluvialis start accumulating high amounts of astaxanthin. As we known, β-carotene ketolase and β -carotene hydroxylase are two key enzymes in the biosynthetic pathway of astaxanthin in H. pluvialis. A variety of environmental stresses can enhance the transcriptional activity of genes encoding for β -carotene ketolase and β-carotene hydroxylase, leading to production of large amounts of astaxanthin in the algae. However, there was still not appropriate approach to understand the regulatory mechanism at a molecular level lack of intact bkt promoter sequences and dependable genetic transformation system in H. pluvialis.