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With the advantages of small volume,high throughput,low consumption and high controllability,droplet microfluidics has shown great potential in the study of single DNA molecule.The development of digital PCR technique taking place in micro-droplet has significantly increased the sensitivity of nucleic acid detection,and provided the feasibility for directed molecule evolution and new-generation sequencing technique.However,existing droplet microfluidics techniques based on water as the reaction carrier are facing some problems,such as the requirement for highly precise syringe pump for droplet generation,high time-consumption for large amount of droplet accumulation,challenge for large-scale real-time droplet monitoring,low capability with other detection device(such as FCM)out of chip and difficulty for droplet storage due to solvent evaporation and droplet fusion.To address the problems,we developed a new droplet generation method by utilizing ultralow-melting-point agarose gel sol-gel transition in a fluorosilane polymers coated PDMS microwell array.More than 200 thousand uniform agarose droplets with controllable size can be generated within 20 s simply by a pressing and a melting steps.In this presentation,the capability of the developed method with digital PCR,single droplet recovery,storage and capability with single droplet manipulation off chip will be demonstrated.