Comprehensive Gene Expression Profiles of both Steady State and Actively Translated mRNA Transcripts

• 作 者: JingfangJu;
• 机 构: Cancer Genomics Laboratory, Assistant Professor of Pharmacology and Medicine, USA-Cancer Research In
• 出 处: 2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
• 发表日期: 2005年期

　　Thymidylate synthase (TS),is a critical target for cancer chemotherapy and is one of the most extensively studied predictive biomarkers for fluoropyrimidine-based chemotherapy.In addition to its critical role in enzyme catalysis,TS functions as an RNA binding protein by regulating its own protein synthesis via a negative autoregulatory mechanism.As an RNA binding protein,TS also regulates the expression of other cellular mRNAs,such as p53 and c-Myc at the translational level.In this study,we investigated the potential genes affected by TS/5-FU treatment at both transcriptional and post-transcriptional levels using human Codelink genome array with a TS-depleted human colon cancer C 18 cell that had been stably transfected with the human TS cDNA.Both steady-state mRNAs and actively translated mRNAs were isolated from control C 18 (TS-),C 18 (TS+) cells and C 18 (TS+) cells treated with 10 mM 5-FU for 4 hr and 24 hr.Gene expression analysis was performed using GeneSpring 7.0 software.