【摘 要】
：
Animal leptospirosis is one of the most common zoonotic diseases in the United States and around the world.In a previous study,we applied four recombinant a
【机 构】
：
DepartmentofPopulationMedicineandDiagnosticSciencesDepartmentofPopulationMedicineandDiagnosticScienc
【出 处】
：
The 5th International Conference on Rodent Biology and Manag
论文部分内容阅读
Animal leptospirosis is one of the most common zoonotic diseases in the United States and around the world.In a previous study,we applied four recombinant antigens,rLipL21,rLoa22,rLipL32 and rLigACon4-8 of L.interrogans for the serological diagnosis of equine leptospirosis (Ye et al,Serodiagnosis of equine leptospirosis by ELISA using four recombinant protein markers,Clin.Vaccine.Immunol.21:478-483) .In this study,the same four recombinant antigens were evaluated for their potential to diagnose canine leptospirosis by ELISA.Canine sera (n= 102) that were Leptospira microscopic agglutination test (MAT) -negative and sera (n=203) that were MAT-positive to the 5 serovars (serovars Pomona,Grippotyphosa,Icterohaemorrhagiae,Canicola and Hardjo) were compared to canine sera (n=102) that were MAT-negative.When individual recombinant antigens were used,the sensitivity and specificity of ELISA were 97.5%and 84.3%for rLigACon4-8; 89.7%and 81.4%for rLoa22; 92.6%and 84.3%for rLipL32 and 99.5%and 84.3%for rLipL21,respectively compared to the MAT.The sensitivity and specificity of ELISA were,92.6%and 91.2%for rLigACon4-8 and rLipL32,97.5%and 84.3%for rLigACon4-8 and rLipL21,89.7%and 87.3%for rLigACon4-8 and rLoa22,89.7%and 87.3%to rLipL21 and rLoa22,92.6%and 91.2%for rLipL21 and rLipL32 and 89.2%and 94.1%for rLoa22 and rLipL32 when one of the two antigens was test positive.The use of all four antigens in the ELISA assay was found to be sensitive and specific,easy to perform,and agreed with the results of the standard Leptospira Microscopic Agglutination test (MAT) for the diagnosis of canine leptospirosis.
其他文献
Stra8 (Stimulated by retinoic acid gene 8) is a marker gene of meiosis,and is regulated by upstream genes on hypothalamic–pituitary–gonadal axis and contr
Peste des petits ruminants (PPR) is an acute and contagious disease of some small ruminants caused by peste des petits ruminants virus (PPRV) .Fusion (F) pr
11 strains of H9N2 Avian influenza virus (AIV) were isolated from the central region of China from 2010 to 2012,amplifying HA gene with RT-PCR and analyzing
A survey of helminths parasitizing the digestive tract of bats in Henan Province was carried out from August 2003 to December 2013.These bats collected from
A number of surface proteins specific to pathogenic strains of Leptospira have been identified.The Lig protein family has shown promise as a marker in typin
From the 1960s to the mid 1990s the dominant paradigm for rodent control was the widespread use of chemical rodenticides.In general,farmers were reactive to
In Myanmar,Bandicota bengalensis is the main rodent pest and is ranked as the second most important constraint to production in lowland rice ecosystems.Prev
Rodents are a key pest to agricultural and rural island communities of the South Pacific,but there is limited information of their impact on the crops and l
In many parts of Europe,common voles (Microtus arvalis) are the main vertebrate problem species in agriculture.During outbreaks populations reach densities
Feral swine could serve as a reservoir of influenza A virus or potentially represent a spatially dynamic "mixing vessel" given their free-ranging habits.Her