【摘 要】
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The B-cell activating factor (BAFF) is a member of the tumor necrosis factor (TNF) superfamily that specifically regulates B lymphocyte proliferation and survival.Excess BAFF also leads to overproduct
【机 构】
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Hebei Key Lab of Laborotary Animal Science, Hebei Medical University, Shijiazhuang, P.R.China
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The B-cell activating factor (BAFF) is a member of the tumor necrosis factor (TNF) superfamily that specifically regulates B lymphocyte proliferation and survival.Excess BAFF also leads to overproduction of antibodies for secretion,anti-dsDNA antibodies and a lupus-like syndrome in mice.To investigate whether transgenic overexpression of the zebrafish BAFF leads to immunoglobulin changes and/or early maturing of the immune system,a Tol2-GFP-2A-BAFF/His recombinant plasmid was constructed by inserting a 2A peptide between the GFP and BAFF sequences.Functional GFP and BAFF proteins were expressed separately and confirmed in HeLa cells.The relative expression of immune-related genes (IgLC-1,IgLC-2,IgLC-3,IgD,and IgM,IL-4),early lymphoid markers (Ikaros,Rag-1,TCRAC),and the proto-oncogene Bcl-2 were evaluated by quantitative PCR in transgenic zebrafish juveniles and adults.Ectopic expression of BAFF in adults was confirmed with western blots and was shown to up-regulate IgLC-1,IgLC-2,IgD,IgM,IgZ/T,Ikaros,Rag-1,TCRAC,IL-4,and Bcl-2 expression in juveniles on day 21 and IgLC-1,IgLC-2,IgD,IgM,IgZ/T,Rag-1,TCRAC,and Bcl-2 expression in zebrafish 3 months post fertilization.Therefore,our findings provided novel insight into further exploration of modulating adaptive immunity and studying autoimmune diseases caused by regulating BAFF.
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