Determination of Mini-short Tandem Repeat (miniSTR) Loci by Using the Combination of Polymerase Chai

• 作 者: XuexiaLIN[1]JingWU[2]HaifangLI[2]ZhihuaWANG[3]Jin-MingLIN[2]
• 机 构: State Key Laboratory of Chemical Resource Engineering,Beijing University of Chemical Technology,Beij
• 出 处: 第十四届国际电分析化学会议
• 发表日期: 2013年期

　　In this work,a simple and convenient method for the detection of mini-short tandem repeat (miniSTR) loci has been developed by the combination of polymerase chain reaction (PCR) and microchip electrophoresis (MCE).Degraded or inhibitor DNA was greatly limited STR loci analysis.The proper primers designed as close as possible to the STRs region to produce smaller size STRs,and made the assay suitable for the destroyed samples.Two annealing temperatures were applied in one PCR procedure and the corresponding cycle numbers were studied to improve the specificity of PCR reaction.The conditions of amplification were investigated for getting a stable and reliable results.Under optimum conditions,0.001ng DNA templates were enough to generate miniSTRs.The relative standard deviations (n=3) of the size fifteen miniSTRs from DNA9947A were between from 0.49% and 4.41%.The RSDs of concentrations were between 0.94% and 4.95%.Fifteen miniSTRs were also well produced from human hair which was exposed in environment made DNA damage,indicating the method has great potential application for criminal identification and paternity testing.