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As a member of the miR106b-25 cluster, miR-93 is reportedly dysregulated in many tumors and acts as a tumor promoter, including overexpression in breast cancer.However,research on its mechanism is not very clear.In our preliminary study, miR-93 expression was lower in human colon cancer tissue and colorectal carcinoma cell lines than in normal colon tissues.This result is not similar to the miR-93 expression found in breast cancer.Therefore, we conducted a series of studies regarding miR-93 effects for colon cancer development.In vitro,miR-93 has an inhibitory effect on colon cancer proliferation in HCT-116 and HT29 cell lines.Furthermore, miR-93 inhibits migration, invasion function, and monoclonal formation ability of the HCT-116 colon cancer cell line.Through the bioinformatics prediction method, SMAD7 and BAMBI were identified as candidate targets for miR-93 expression.SMAD7and BAMBI were considered as the main molecules in the TGF-β and WNT and BMP signaling pathways, whose activation has an important function on colon cancer development.Further molecular mechanism study on miR-93 is underway.