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This study was aimed to assess remyelination and axon regeneration of compounds using Hunter Biotech s zebrafish MS models.Zebrafish at 2 day post fertilization (2 dpf) were treated with demyelinating agent ethidium bromide (EB) for 72 hours (hrs) to induce demyelination.After removing EB, demyelinated zebrafish were treated with test compounds for another 24 hrs; Compound effect on demyelinationassociated motility was assessed using a videotrack motion detector.We found that compounds 2 and 9 recovered motility in EBinduced demyelinated zebrafish ; whereas compounds 2 and 9 had significant effect on motility.Compounds 2 and 9 were confirmed by whole mount antimyelin basic protein (antiMBP) immunostainging, further supporting that demyelinationassociated motility assay (primary assay) in combination with Fluoro Myelin staining (secondary assay) is a reliable array of methods for relatively high throughput in vivo remyelinating drug screening and assessment.