【摘 要】
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Background: Quantification of polar compounds such as chloroquine by revered-phase liquid chromatography is a challenge because of poor retention and silano
【机 构】
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The Affiliated Brain Hospital of Guangzhou Medical University(Guangzhou Huiai Hospital),Guangzhou 51
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Background: Quantification of polar compounds such as chloroquine by revered-phase liquid chromatography is a challenge because of poor retention and silanol interactions with stationary phase.Strong ion-pairing reagents added to mobile phases to improve reversed-phase retention and improve peak shape can be harmful for mass spectrometry.Results: This new approach provides a rapid and sensitive method for the detection of chloroquine using hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry(HILIC-MS/MS).Ammonium formate and formic acid were added to mobile phase to attain good peak shapes and the salified chloroquine as well retained in an HILIC column.Linearity,intra-and inter-day precision,accuracy,recovery,matrix effect and stability were evaluated during the validation process.Conclusion: The validated method has been successfully used in a pharmacokinetic study in miniature pigs.
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