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This study was to investigate the fractional anisotropy (FA) and mean diffusion (MD) values of diffusion tensor imaging (DTI) in the whole-brain voxel-based analysis of optic neuritis (ON) patients and examine their relationship with visual evoked potentials.A total of 12 patients with ON and 12 healthy controls (HCs) underwent magnetic resonance imaging (MRI).Imaging data were analyzed using two-sample t-tests to identify group differences in FA and MD values.Compared with HCs,ON patients exhibited significantly decreased FA in the left cerebellum posterior lobe,left superior temporal gyrus,left extra-nuclearl,fight middle frontal gyrus,and left middle frontal gyrus and increased FA in the right cerebellum_crus,right lentiform nucleus,bilateral anterior cingulum,left extra-nuclear2 and left precuneus.