【摘 要】
：
氮寨是植物生长发育所必需的大量元素之一.研究植物吸收利用NO3-的规律和机制,对于有效提高作物的氮素利用率具有重要的理论指导意义.本研究通过正向遗传学的方法克隆了
【机 构】
：
山东农业大学生命科学学院,作物生物学国家重点实验室,泰安271018华中农业大学生命科学技术学院,作物遗传改良国家重点实验室,武汉430070SectionofCellandDevelopmentBi
论文部分内容阅读
氮寨是植物生长发育所必需的大量元素之一.研究植物吸收利用NO3-的规律和机制,对于有效提高作物的氮素利用率具有重要的理论指导意义.本研究通过正向遗传学的方法克隆了一个新的NO3-调控基因NRG2.研究发现,nrg2突变体中NO3-响应基因的诱导量明显受到抑制,全苗和根中的NO3-含量显著低于野生型,通过进一步研究发现突变体中NO3-含量的变化可能主要与NRT1.1基因表达量降低及NRT1.8基因表达量增加有关.分子和遗传学分析发现,在NO3-信号调控通路中NRG2位于NRT1.1基因的上游并调控NRT1.1的表达.转录分析结果表明,nrg2突变体中有4个与氮素响应、转运等有关的基因簇发生了改变,多个与氮素相关的下游基因受到NRG2与NRT1.1的共同调控,进一步表明NRG2与NRT1.1在同一条通路中调控NO3-信号.另外,研究发现NRG2与NLP7在细胞核中存在互作关系.
其他文献
How to increase rice productivity to meet the serious challenge of the world population expansion bothers breeders around the world.Here,we report that a ne
Anthocyanin pigmentation is an important consumer trait in peach (Prunus persica).In this study,the genetic basis of the blood-flesh trait was investigated
Photoperiodism is a rhythmic change of sensitivity to light,which helps plants to adjust flowering time accor to seasonal changes in day length and to adapt
Developing wheat varieties with improved ability to use nitrogen (N) efficiently is very desirable,and may offer a sustainable solution to improve crop yiel
Low The survival of seed plants in natural environments requires the successful emergence from the soil.In this process,the ethylene signaling pathway is ut
Potassium and nitrogen are essential macronutrients for plant growth and crop yields.Although the previous studies have indicated that the absorption and tr
MicroRNAs (miRNAs) are noncoding RNAs that are master regulators of various biological processes by post-transcriptionally repressing their target genes.It