MMP-2 contributes to vascular angiogenesis induced by hypoxia/15-HETE in pulmonary arterial endothel

来源 :中国病理生理学会第十届全国代表大会 | 被引量 : 0次 | 上传用户:jy02324491
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AIM:To investigate the role of 15-hydroxyeicosatetraenoic acid(15-HETE) on MMP-2 expression and the role of MMP-2 in pul-monary arterial endothelial cell(PAEC) proliferation and vascular angiogenesis,and to determine whether hypoxia-induced 15-HETE may affect the expression of tissue inhibitor of metalloproteinase-2(TIMP-2).METHODS:The expression of MMP-2 in hypoxic PAECs was detected by Western blotting,immunofluorescence and RT-PCR.The roles and the relationship of MMP-2 and 15-HETE in hypoxia PAECs were analyzed by observing cell viability,BrdU cell proliferation assay and flow cytometry.
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