A microfluidic platform for differential control of the living environment of an embryo

• 作 者: ZeqingBai[1]HongcunBao[2]XiaohangYang[2]MinWang[1]
• 机 构: Institute of Microanalytical Systems,Department of Chemistry,Zhejiang University,Hangzhou 310058,Chi
• 出 处: 第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
• 发表日期: 2014年期

　　Microfluidic techniques open up new opportunities for manipulating the living environment of micron-sized organisms,such as Drosophila embryo,zebra fish and nematode,at high-resolution.In this work,a microfluidic system capable of developing a Drosophila embryos anterior and posterior halves controlled at different temperature was established to study the embryos reaction under environmental perturbations.The microfluidic device was coupled to a confocal microscopy for real-time image acquisition.It was found that the embryo can counteract the effects of these unnatural environmental conditions within each development cycle.