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The aim of the study is to determine the suitable protoscolices (PSCs) density for long-time culturing in vitro.The PSCs were divided into eight groups which densities were adjusted to about 400, 600, 800, 1000, 1200, 1600, 1800, 2000, 2200 PSCs/ml with RPIM 1640 culture medium containing 100 U/ml penicillin, 100 μg/ml streptomycin, 10% calf serum at 37 ℃and 5% CO2.Then the viability tests were carried out by day 3, 4, 5, 6, 7, 8, 9, 14, 22 with 0.1% methylene blue staining.During 22 days of culture,the numbers of live PSCs in each experimental group reduce as the time extension and the viability was quite different among groups.PSCs sustained best at 1800 PSCs/ml with slightly decline in percentage survival which was higher than 80%.Apart from 1600, 1800 and 2000 PSCs/ml groups, other groups showed no PSCs survived at the end of observing period.Though the percentage of live PSCs at the density of 1600 PSCs/ml and 2000 PSCs/ml were higher than 70% during the first 2 weeks of culture,but obviously decreased to 32.8% and 41.5% respectively after another one week culture.The results of this study suggested that the density of PSCs culturing is an important factor for extending the culturing time of PSCs in vitro and about 1800 PSCs/ml was a favorable density in the culture system used in this experiment.