【摘 要】
：
Tobacco smoking, a widely spread habit, has been found associated with declined cognitive functions.Fasudil, an inhibitor of Rho-associated coiled-coil kinase, possesses a strong neuroprotective poten
【机 构】
：
School of Pharmaceutical Sciences, South-Central University for Nationalities,Wuhan 430074, PR China
【出 处】
：
中国民族医药学会药品临床评价分会2016年学术年会
论文部分内容阅读
Tobacco smoking, a widely spread habit, has been found associated with declined cognitive functions.Fasudil, an inhibitor of Rho-associated coiled-coil kinase, possesses a strong neuroprotective potential.The current study was designed to investigate the pathological changes in brain and lung induced by smoke exposure, and explore whether fasudil could alleviate these impairments.Adult C57BL/6 mice were exposed to tobacco smoking for four months, and fasudil was treated from the third months.To investigate lung injuries, the immunohistochemistry of lung tissue, immune cell infiltrations, cytokine productions in bronchoalveolar lavage (BAL) fluid, and seurm inflammatory cytokines were evaluated.To investigate cognitive impairments, Morris water maze test, hippocampal inflammatory cytokines and associated signaling pathways were evaluated.Out finding showed fasudil administration inhibited the inflitration of inflammatory cells (macrophages, neutrophils, and lymphocytes), suppressed the production of inflammatory cytokines both in the BAL fluid and serum.Further, fasudil treatment significantly improved the spatial learning and memory impairments as indicted in Morris water maze test and reduced the elevation of hippocampal inflammatory cytokines induced by tobacco smoking.Of note,expressions of RhoA, ROCK1, ROCK2, caspase-3, caspase-9, bax and the phosphorylation of NF-kBp65, IkBα, IKKα and IKKβ were increased accompanying the smoke exposure-induced cognitive impairments, which were significantly restored by fasudil treatment as indicted in western blot and immunohistochemistry analysis.Together, our results showed that fasudil exhibited protective effects on smoke exposure induced cognitive deficits which might involve with the regulation of Rho/ROCK/NF-kB pathways.
其他文献
Objective: To investigate the effects of icariin (ICA) on the proliferation of pulmonary artery smooth muscle cell (PASMC) induced by endothelin-1 (ET-1), and explore its mechanisms.Methods: PASMC fro
Objective: To investigate the protective effects of icariin (ICA), one of active components of epimedium, on Lipopolysaccharide (LPS)-induced dopaminergic (DA) neuronal damage in rats.Methods: Male ra
抑郁症是由多种因素引起的情感障碍性疾病，其特点是发病率高、致死率高和复发率高，给社会带来了严重的经济负担。抑郁症的发病机制复杂，现有研究表明其发病机制主要涉及单胺递质、下丘脑-垂体肾上腺轴功能失调、神经再生障碍和表观遗传学等，但是针对这些机制的治疗药物缺乏治疗效果并伴有副反应。因此，进一步研究抑郁症的发病机制至关重要。
Icariside Ⅱ (ICS Ⅱ) is a natural product extracted from Herba Epimedii Maxim and possesses diverse biological activities including antineoplastic effects.Recently, the role of ICS Ⅱ has been extensive
Object: Myocardial fibrosis is the endpoint pathology common to many cardiovascular disorders.We have previously shown that apocynin (APO), a naturally occurring NADPH oxidase inhibitor, significantly
目的:观察金钗石斛总生物碱(Dendrobium nobile Lindl.Alkaloids,DNLA)对db/db小鼠血糖的影响,并探讨其可能的作用机制.方法:雄性C57BL/KsJ小鼠随机分为正常组、正常给药组(DNLA 80 mg/kg),雄性db/db小鼠分为模型组、DNLA治疗组(20、40、80 mg/kg)、RSG阳性药对照组(2 mg/kg)及联合用药治疗组(RSG 2 mg/k
目的：探讨中医治疗冠心病的用药经验.方法：以中医治疗冠心病为背景,收集中医治疗冠心病的病案,采用关联规则apriori算法、复杂系统熵聚类等无监督数据挖掘方法,确定处方中各种药物的使用频次及药物之间的关联规则,挖掘核心组合,分析中医治疗冠心病的用药经验.结果：处方常用药物包括当归、丹参、川芎、黄芪、红花、赤芍、桃仁、茯苓、生地黄、甘草、麦冬、郁金、五味子、桂枝、柴胡、白芍、薤白、瓜蒌等,这些药物多
目的：系统评价双黄连注射剂治疗病毒性脑膜炎的有效性及安全性.方法：计算机检索中国期刊全文数据库CNKI,万方数据库,维普数据库VIP,PubMed和Cochrane Library等数据库,全面收集双黄连注射剂治疗病毒性脑膜炎的随机对照试验(RCT)的文献,检索时间段设定均为建库日期至2015年12月.采用Cochrane风险评价表进行文献质量评价,提取资料并运用Revman5.2软件进行Meta
目的：探讨中药黄连的主要药用成分小檗碱诱导人结直肠癌细胞自噬以及自噬性细胞死亡的机制.方法：不同浓度的小檗碱作用于人结直肠腺癌(HT-29)细胞后,用MDC方法以及免疫荧光方法观察细胞自噬的高低;MTT方法检测小檗碱对细胞增殖的影响;免疫印迹法检测小檗碱对人结直肠癌细胞自噬相关的信号通路的影响.结果：;MDC方法以及免疫荧光染色显示,随着不同浓度的小檗碱处理细胞24小时以及50μmol/L的小檗碱
目的：系统评价双黄连注射剂治疗手足口病的有效性及安全性.方法：计算机检索中国期刊全文数据库CNKI,万方数据库,维普数据库VIP,PubMed和Cochrane Library等数据库,全面收集双黄连注射剂治疗手足口病的随机对照试验(RCT)的文献,检索时间段设定均为建库日期至2015年12月.采用Cochrane风险评价表进行文献质量评价,提取资料并运用Revman5.2软件进行Meta分析.结