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Brassinosteroids (BRs) are essential steroid hormones that regulate plant growth and development.The transcription factor BRI1-EMS-SUPPRESSOR1 (BES1) regulates the expression of thousands of target genes in response to BRs.Here we report an Arabidopsis thaliana-specific long isoform of BES 1,BES l-L,which has stronger activity in promoting BR signaling than the canonical and widely used short BES 1-S.