Detection of Effects of knocking-down Nueleostemin gene on apoptosis of HL-60 ceHs in vitro

• 作 者: BaohongYue[1]YuanyuanWang[1]LinaYu[1]ShuzhenFu[1]XiaoliSun[1]QinxianZhang[2]QuanchengKan[3]
• 机 构: Department of Clinical Laboratory Medicine,The First Affiliated Hospital,Zhengzhou University
• 出 处: 中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
• 发表日期: 2011年期

@@ Nucleostemin，which exists in stem cells and some malignant tumor cells，is highly expressed in various kinds of tumor cells such as leukemia cells.NS may play a significant role in the self-renewal and infmite proliferation state of stem cells and malignant tumor cells，and keeps cells in non-difierentiated state.To explore whether the apoptosis of leukemia cells could be induced by knocking—down Nucleostemin(NS)gene in vitro. The totallength of the synthesized NS-shRNA fNucleostemin specific short hairpin