论文部分内容阅读
Introduction: Zirconia ceramics have been introduced into dentistry as a metal replacement for implant,but its bioinertness causes many problems.It is necessary to improve its biological activity.Materials and Methods: The pre-sintered zirconia discs(13 mm in diameter and 1.5 mm in thickness)were randomly divided into 4 groups: Z0 as control group,Z1,Z2 and Z3 were immersed in the Ca-P precursor solution for 1,2 and 3 minutes,respectively.After dense sintering,another 4 subgroups was divided from above 4 groups named as Z0h,Z1h,Z2h and Z3h,which were hydrothermal treated.Morphology and composition of these groups were investigated by Scanning Electron Microscopy and Energy Dispersive Spectrometer; Cross-section morphology of group Z2 was evaluated with SEM.In vitro osteoblast-like cell(MC3T3-E1)was used to evaluate the cell proliferation kinetic and ALP activity of the groups; Statistical analysis was performed using an ANOVA test,the results were taken to be significant at a probability level of P<0.05.Results: Experimental groups showed Ca-P clusters on their surfaces,and the distribution densities of clusters were increased with the extension of immersion time.Ca-P clusters turned into rod crystals after hydrothermal treatment.The EDS data showed a molar ratio about 1.67 at the area of rod crystals in hydrothermal treated groups; Ca-P clusters infiltrate into zirconia with a depth of approximately 8 um and crossed each other.The cell morphology changing to flat was observed on group Z2h at 24h cultivation,the cell proliferation on group Z2h was significantly higher than group Z2 at 7 days,the cell ALP activity of Z2h was high significantly compared with that of Z0 and Z2 at 7,14 days(p<0.05).Conclusions: This studies offers an effective way to modify the zirconia surface to improve its biocompatibility.