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Objective: To construct two vectors expressing α-Fodrin and investigate its treatment on mice model with primary Sjogrens syndrome (NOD mice).Methods: Eight-week-old NOD mice were randomly divided into four groups, such as control group, empty vector group, α-fodrin-siRNA1 group and α-Fodrin-siRNA2 group, and there are 4 rats in each group.Four template DNA of α-Fodrin siRNA were chemically synthesized and annealed to two dsDNA, then digested by BamH Ⅰ and Hind Ⅲ.The digested double strands oligos were inserted into the downstream of U6 promoter of linearized pGFP-V-RS vector.Recombinant were confirmed by restrictive enzyme digestion and sequencing.Then the successful vector were injected by tail veil into NOD mice,while rats in the control group were injected with the same dose of PBS and empty vector group were injected with the same dose of empty vector.Serum IFN-γ, IL-17 concentrations in each group were detected by ELASA in order to observe changes in cytokine levels.At the same time,the pathological changes of the lacrimal gland and organ with HE staining were observed α-Fodrin mRNA in lung were detected by RT-PCR.Results: (1) We constructed two siRNA eukaryotic expression vector successfully;(2) Compared with the control group and empty vector group,serum IFN-γ, IL-17 levels in treatment groups were significantly decreased (p<0.05), but IFN-γ levels in α-Fodrin-siRNA groups decreased little after 24 hours (p>0.05).(3) Compared with the control group and empty vector group, lymphocyte infiltration of lacrimal gland and inflammatory cell infiltration of alveolar and interstitial were significantly reduced in α-Fodrin-siRNA groups;(4) Compared with the control group and empty vector group, the expression ofα-Fodrin mRNA in lung were significantly reduced in α-Fodrin-siRNA groups.Conclusion: Sepecial α-fodrin siRNA can supress the involvment of lacrimal glands and lung, also inhibit the inflammation in mice with primary sjogrens syndrome.So the constructed vectors may improve the progression of pSS.