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目的:先前的研究证明HMME-PDT能显著灭活HIV-1ⅢB,本实验进一步研究各组成因素对HMME-PDT灭活HIV效应的影响以及HMME-PDT对多种HIV病毒株的灭活效应,探讨其临床应用的可能。方法:分别采用合胞体计数法、MTT法和捕捉HIV-1p24抗原ELISA法检测光敏剂浓度、能量密度、功率密度以及抗
AIM: Previous studies demonstrated that HMME-PDT can significantly inactivate HIV-1ⅢB. This study further investigated the effect of various factors on the inactivation of HIV by HMME-PDT and the inactivation effect of HMME-PDT on various HIV strains Its clinical application is possible. Methods: The concentrations of photosensitizer, energy density, power density and anti-HIV antibody were detected by syncytium count, MTT assay and HIV-1 p24 antigen ELISA