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目的:观察中药劲男春颗粒对老龄大鼠阴茎海绵体组织氧化应激损伤的保护作用,为临床推广应用提供理论依据。方法:将45只雄性老龄SD大鼠随机分为劲男春颗粒组、疏肝益阳胶囊组、空白组,每组15只。各组分别给予0.9%氯化钠注射液、疏肝益阳胶囊[1 g/(kg·d)]及劲男春颗粒[8 g/(kg·d)]。给药4周后检测各组大鼠阴茎海绵体组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)的含量。结果:劲男春颗粒组大鼠阴茎海绵体的SOD活性显著高于空白组(P<0.01),MDA含量低于空白组(P<0.05),NO含量和NOS活性高于空白组(P<0.05);疏肝益阳胶囊组大鼠阴茎海绵体的NO含量及NOS活性显著高于空白组(P<0.01),MDA含量低于空白组(P<0.05),SOD活性高于空白组(P<0.05);劲男春颗粒组与疏肝益阳胶囊组的SOD、MDA、NO及NOS浓度比较,差异均无统计学意义(P>0.05)。结论:劲男春颗粒对老龄大鼠阴茎海绵体组织氧化应激损伤具有保护作用,通过拮抗阴茎海绵体的氧化应激损伤可能是劲男春颗粒治疗老年性勃起功能障碍的作用机制之一。
OBJECTIVE: To observe the protective effect of Jinnanchun Granule on oxidative stress injury of the corpus cavernosum in aged rats, and provide a theoretical basis for clinical application. Methods: Forty-five male SD rats were randomly divided into Jinnanchun granule group, Shuganyiyang capsule group and blank group, with 15 rats in each group. Each group was given 0.9% sodium chloride injection, Shugan Yiyang capsule [1g / (kg · d)] and Jinnanchun particles [8g / (kg · d)]. Four weeks after administration, the contents of superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) and nitric oxide synthase (NOS) in the corpus cavernosum of rats in each group were measured. Results: The SOD activity in penis of Jinnanchun granule group was significantly higher than that in the blank group (P <0.01), MDA content was lower than that in the blank group (P <0.05), NO content and NOS activity were higher than those in the blank group (P < 0.05). The content of NO and the activity of NOS in the penis of Shugan Yiyang capsule group were significantly higher than those in the blank group (P <0.01), while the content of MDA was lower than that in the blank group (P <0.05) <0.05). There was no significant difference in SOD, MDA, NO and NOS concentrations between Jinnanchun granule group and Shuganyiyang capsule group (P> 0.05). CONCLUSION: Jinnanchun Granule has a protective effect on the oxidative stress injury of the corpus cavernosum in aged rats. Antagonistic effect of Jinnanchun Granules on senile erectile dysfunction may be one of the mechanisms by which antagonizing the oxidative stress injury of the penis cavernous.