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Objective: To investigate the effects of growth factors fibroblast-derived extracellular matrix formation in the 3D fiber-deposited polycaprolactone(PCL)scaffold fabricated by 3D printing technique for tissue engineering applications of tunica albuginea.Methods: PCL scaffold was fabricated by 3D bioprinting system.For in vitro cell study,scaffolds were seeded with human fibroblast cell at 5×105 cells and were cultured for up to 2 weeks.Cell survival and cell proliferation were monitored by EZ-cytox assay.The effect of growth factors on the extracellular matrix formation was evaluated by fastin elastin assay and enzyme immunoassay(EIA).Results: SEM images showed the surface morphology of PCL scaffolds.Human fibroblasts were grown on 3D PCL scaffolds in the presence/absence of basic fibroblast growth factor(bFGF)or transforming growth factor-beta 1(TGF-β1).bFGF or TGF-β1 stimulated proliferation of fibroblasts and also increased collagen and elastin formation in vitro study.Conclusions: This study shows that bFGF or TGF-β1 modulates the fibroblast-derived extracellular matrix formation in the 3D PCL scaffold.