【摘 要】
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Objective: Metallothionein (MT) is a small,cysteine-rich,metal-binding protein that plays an important role in protecting against toxicity of heavy metal and ch
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Objective: Metallothionein (MT) is a small,cysteine-rich,metal-binding protein that plays an important role in protecting against toxicity of heavy metal and chemicals.This study was aimed to define diurnal variation of MT in mice.Methods: Adult mice were maintained in the SPF-grade animal facilities for 2 weeks with light on at 8:00 and light off at 20:00.The liver and kidneys were collected every 4 h during the 24 h period.Total RNA was isolated,purified,and subjected to real-time RT-PCR analysis.Results: The diurnal variations in mRNA levels of MT-1 and MT-2 in liver were dramatic,up to 40-fold of peak/trough ratio.MT mRNA levels in kidneys also showed diurnal variation,up to 7-fold of peak/trough ratio.The diurnal variation of MT mRNAs resembled the clock gene albumin site D-binding protein (Dbp) in liver and kidneys.Conclusion: MT mRNA levels showed diurnal variations in the liver and kidneys in mice,which could impact body defense against toxic stimuli.
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