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Surface assisted laser desorption/ionization mass spectrometry has been developed as a promising technique,which affords several advantages such as the ability to detect small molecules,easy sample preparation,low-noise background and so on[1,2].However,to elucidate the impact of surface features on the desorption/ionization performance is still a great challenge because of the random morphologies of the substrate and the complexity of desorption/ionization process[3].Recently,we demonstrated a method to fabricate biomimetic silicon nanocones array with the nanosphere masked reactive ion etching for suppressing the reflection[4].In this work,biomimetic silicon nanocones arrays,which could be applied for improving the desorption/ionization performance due to the high photo-absorption ability,are fabricated and used as laser desorption/ionization substrate.Surface features such as the height and period of the silicon arrays can be controlled precisely,so that their impact on the desorption/ionization performance is investigated systematically.The optimized silicon array exhibits excellent performance for detecting peptides,amino acids and polymers without any interference in the low-mass region.Furthermore,the application of this method is demonstrated by the successful analysis of glucose in urine sample from a diabetic patient.