The methodology of constructing expression cassettes in synthetic biology

来源 :2013 International Symposium on Biocatalysis and Biosyntheti | 被引量 : 0次 | 上传用户:kfanliang127
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  Studies in the structural biology of the multi-component protein complex,metabolic engineering and synthetic biology frequently rely on the efficient over-expression of these subunits or enzymes in the same cell.
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Toexpress homologous or heterologous proteins in fungi,a protein expression system using thepromoterof cellobiohydrolase Ⅱ gene(cbhⅡ)wasconstructedby generating an expression vector called pWEIIF00.
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