【摘 要】
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Objective: To Study on Increasing Efficacy and Reducing Toxic Substance of Angelica Sinensis and Sophorae Flavescentis ait Pill (ASSF) combined with cisplatin (DDP) on H_ (22) tumor-bearing mice.Metho
【机 构】
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Gansu Traditional Chinese Medicine College, Lanzhou 730020;Key Laboratory for Molecular Medicine of
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Objective: To Study on Increasing Efficacy and Reducing Toxic Substance of Angelica Sinensis and Sophorae Flavescentis ait Pill (ASSF) combined with cisplatin (DDP) on H_ (22) tumor-bearing mice.Methods: Bodyweight,tumor inhibition rate and q value were calculated for single ASSF or ASSF combined with DDP on H22 carcinoma xenograft KM mice;biochemical method for serum detection of LDH, AST, ALT, AKP,ELISA method for serum HIF-1 α, pathological detection of thymus, immunohistochemistry detection of tumor tissue caspase3 and mutant p53 protein, and qRT-PCR detection of bax/bcl-2 mRNA were applied.Results:Compared with DDP control group, the bodyweight increased in ASSF-DDP group (p<0.01).Tumor inhibition rates for DDP, ASSF, ASSF-DDP were 62.7%.43.7% and 71.0% respectively.q value was 0.90.Compared with other groups, thymus of DDP coutrol group had obvious pathological injury (p<0.01), serum LDH, AST,ALT, AKP increased significantly in DDP control group (p<0.0l), serum HIF-1 α increased in model control group.Compared with model control group, the expression of mutant p53 protein and bcl-2 mRNA decreased in all treatment groups (p<0.01), but there were no statistical difference between DDP control group and ASSF-DDP group, the expression of caspase3 protein and bax mRNA increased in all treatment groups, and there were statistical difference between DDP group and ASSF-DDP group (p<0.01).Conclusion: ASSF can inhibit bodyweight decrease caused by DDP, can inhibit tumor growth synergistically with DDP mainly through increasing serum HIF-1 α and pro-apoptotic molecules such as caspase 3 and bax, not through decreasing anti-apoptotic mutant p53 and bcl-2.ASSF can reduce DDP toxicity due to decreasing the release of LDH,AST, ALT, AKP into blood and enhancing thymus protection.
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