Mechanisms of radiosensitization induced by Tat-SmacN7 in tumor cells

• 作 者: WANGYan;FANFeiyue;FANSaijun;DULiqing;XUChang;CAOJia;WANGQin;CHENFenghua;FUYue;GUOYanting;LIUQiang;
• 机 构: Tianjin Key Laboratory of Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy
• 出 处: 第二届全球华人辐射研究大会
• 发表日期: 2014年期

　　To explore the mechanisms of Tat-SmacN7 in radiosensitization by the activation of caspases and induction of apoptosis pathway in EC109 and NCL-H460 cancer cells.The cells are divided into four groups: the control group,the Tat-SmacN7 group,the radiation group,the combined Tat-SmacN7 with radiation group.The transcriptional level of caspase-3,-8 and -9 were fitted by RNA extraction,cDNA synthesis and quantitative real-time PCR.The activity of caspase-3,-8 and-9,was analyzed using a fluorogenic caspase assay with Ac-DEVD-AFC,Ac-IETD-AFC,Ac-LEHD-AFC as substrates,respectively.z-VAD-fmk treatment completely blocked apoptotic combination of Tat-SmacN7 and radiation in both EC109 and H460 cells.The activity and mRNA expression of caspase-3,-8 and -9 were found increased significantly in Tat-SmacN7 group.Tat-SmacN7 increases tumor radiosensitivity through mitochondria pathway of apoptosis,which is expected to be a new radiosensitization agent in the future.