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Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise,targeted changes to the genome of living cells.CXCR4 is a co-receptor for the human immunodeficiency virus type 1(HIV-1)infection and has been considered as an important therapeutic target for AIDS.CXCR4 mediates viral entry into human CD4+cells by binding to envelope protein,gpl20.Here,we show that human CXCR4 gene is efficiently disrupted by CRISPR/Cas9-mediated genome editing,leading to HIV-1 resistance of human primary CD4+ T cells.