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Metal ions operate as cofactors for around 40％enzymes,however,they also exhibit toxic effects.It is crucial to identify metal-protein interactions at a proteome-wide scale(1)which are difficult due to some weak and transient interactions.We developed an integrated approach consisting of gel electrophoresis and inductively coupled plasma mass spectrometry,LA-ICP-MS,IMAC,fluorescence and bioinformatic approach to identify metal-associated proteins using bismuth antiulcer drug and silver as an example(2,3).