CARBON NANOFIBER EMBEDDED PLGA CARDIOVASCULAR BIOMATERIAL FOR INCREASED CARDIOMYOCYTE GROWTH

• 作 者: David Stout
• 出 处: 第六届海内外中华青年材料科学技术研讨会暨第十五届全国青年材料科学技术研讨会
• 发表日期: 2015年6期

　　During a heart attack,the hearts oxygen supply is cut off,and eardiomyocytes perish.Unfortunately,once these tissues are lost,they cannot be replaced and results in cardiovascular disease-the leading cause of deaths worldwide.Advancements in medical research have been targeted to understand and combat the death of these cardiomyocytes.For example,new research(in vitro)has demonstrated that one can expand cardiomyocyte adhesion and proliferation using poly-lactic-co-glycolic acid(PLGA)(50:50(weight percent))supplemented with carbon nanofibers(CNFs)to create a cardiovascular patch.However,the examination of other cardiovascular cell types has not been investigated.Therefore,the purpose of this present in vitro study was to determine cell growth characteristics of three different important cardiovascular cell types(aortic endothelial,fibroblast and cardiomyocyte)onto the substrate.Cells were seeded onto different PLGA:CNF ratio composites to determine ifCNF density has an effect on cell growth,both in static and electrically stimulated environments.During continuous electrical stimulation(rectangle,2 nm,5 V/cm,1 Hz),cardiomyocyte cell density increased in comparison to its static counterparts after 24,72 and 120 hours.A minor rise in Troponin Ⅰ excretion in electrical stimulation compared to static conditions indicated nominal cardiomyocyte cell function during cell experiments.Endothelial and fibroblast cell growth experiments indicated the material hindered or stalled proliferation during both static and electrical stimulation experiments,thus supporting the growth of cardiomyocytes onto the dead tissue zone.Furthermore,the results specified that CNF density did have an effect on PLGA:CNF composite cytocompatibility properties with the best results coming from the 50:50 [PLGA:CNF(weight percent:weight percent)] composite.Therefore,this study provides further evidence that a conductive scaffold using nanotechnology should be further research for various cardiovascular applications.