Determination of parabens in cosmetics by subcritical water chromatography with salt-assisted homoge

• 作 者: Hou-KuangShih;Chiao-WenLin;Jen-FonJen;
• 机 构: Department of Chemistry, National Chung-Hsing University, Taichung 402, Taiwan
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　A green analytical method for the determination of parabens in cosmetic products by subcritical water chromatography (SbWC) with salt-assisted homogenous liquid liquid extraction (SHLLE) was investigated.After a dilution of cosmetic sample with water, parabens in 5 mL aqueous sample solution (adjusted pH to 6) were extracted into 200 μL isopropanol after adding 4 g of ammonium sulfate in a self-designed glass extraction device.The extractant was collected, dried by nitrogen purging, and re-dissolved in 10 μL of water (pH 10.5), prior to subcritical water chromatographic separation and followed by UV detection.After examining the parameters affecting the performance of SbWC, the best results for the SbWC were obtained by using PLRP-S (100(A) 5μm, 15 cm × 4.6 mm I.D.) column with pH 10.5 citric acid buffer as mobile phase with the temperature of preheater and column oven as 80℃ and 100℃ respectively.